clinical grade human adipose tissue-derived mesenchymal stem cell banking
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abstract
in this study, our aim was to produce a generation of gmp-grade adipose tissue-derived mesenchymal stem cells for clinical applications. according to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cgmp) compliant adipose tissue-derived mesenchymal stem cells from five female donors. the isolated cells were cultured in dmem supplemented with 10% fetal bovine serum and characterized by standard methods. moreover, karyotyping was performed to evaluate chromosomal stability. mean of donors’ age was 47.6 ± 8.29 year, mean of cell viability was 95.6 ± 1.51%, and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106 ± 2863564.21 per microliter. the main aim of this project was demonstrating the feasibility of cgmp-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials.
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Journal title:
acta medica iranicaجلد ۵۳، شماره ۹، صفحات ۵۴۰-۵۴۶
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